Boll weevil gene flow estimates between the Valley and the Kingsville area
| Problem: | |
| Objective: | Determine the level of boll weevil migration between the Lower Rio Grande Valley and the Southern Coastal Bend across the potential dispersal barrier imposed by the King Ranch |
| Approach: | Conduct RFLP marker analysis of boll weevils collected during the summer of 2002 from multiple locations just south of Kingsville, TX, and from Weslaco, TX. Isolate the genomic DNA, and after verification of DNA quality by electrophoresis, perform long-PCR. Adequate amplification is checked for each sample by electrophoresis, and those that were successfully amplified are cut with restriction enzymes. The cut samples are saved for later separation by electrophoresis. After completion of the laboratory work in Weslaco, Dr. K.-S. Kim will analyze the data and draw conclusions about the level of weevil migration occurring between the Valley and the Southern Coastal Bend across the potential dispersal barrier imposed by the King Ranch. |
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Significant Impact: |
K.-S. Kim, Tom Sappington